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| Sample Submission Guide |
Macrogen has served over 10 years in sequencing
field using the cutting edge technology and
delivering fast and reliable results. We
use state-of-the-art high throughput Applied
Biosystems 3730XL sequencers.
We sequence all kinds of cloned DNAs (plasmids,
cosmid, phages, BACs) as well as PCR-products.
Macrogen provides a high quality DNA Sequencing
service for academics, government, research
institutions and private companies at Very
Competitive Price.
Please contact us for any inquiry.
For more detailed explanations and recommendations
about the preparation and submission of samples,
please refer to the following link:
To order the sequencing service, please
access to our online ordering system and create an account;
http://dna.macrogen-singapore.com/eng
For payment information, please refer
to the following link:
 Download 'Payment Information'
http://dna.macrogen-singapore.com/eng/account/guide/howtopay.jsp
Contact
Sequencing Inquiry: info-sg@macrogen.com
Payment Inquiry: payment-sg@macrogen.com
Genome and Project sequencing inquiry: ngs.apac@macrogen.com
Technical Support
Macrogen-Korea : Macrogen-Korea
Macrogen-Europe : Macrogen-Europe
Macrogen-Spain : Macrogen-Spain
Macrogen-Singapore : Macrogen-Singapore
Shipping Address
(for Value Service to Macrogen Inc.)
Macrogen Inc.
#1001, 254 Beotkkot-ro,
Geumcheon-gu, Seoul,
SEOUL 08511 Rep. of KOREA
Tel : +82-2-2113-7054
Fax : +82-2-2180-7100
E-mail : info-sg@macrogen.com
Shipping Address (For
European Express Service to Macrogen Europe)
Macorgen Europe
Meibergdreef 31
1105 AZ, Amsterdam
the Netherlands
Tel : +31-20-333-7563
E-mail : europe@macrogen.com
Shipping Address (For Spanish, Portuguese and Italian Express Service to Macrogen Spain)
Macorgen Spain
Avda Sur del aeropuerto de Barajas 28, PB
28042 Madrid
Tel : +34 911 138 378
E-mail : info-spain@macrogen.com
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 Prepare samples and primers
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| Template/Service |
Concentration |
Sample Volume |
Primer Volume
+ Concentration |
Plasmid
(below 4kb ) |
100~150ng/ul |
5ul for 1st reaction + 5ul for re-trial reaction |
5ul + 5pmol/ul |
Plasmid
(over 4kb) |
200ng/ul |
5ul for 1st reaction + 5ul for re-trial reaction |
5ul + 5pmol/ul |
PCR product(below 200bp)
Purified / Unpurified |
10ng/ul |
5ul for 1st reaction + 5ul for re-trial reaction |
5ul + 5pmol/ul |
PCR product(201bp~500bp)
Purified / Unpurified |
20ng/ul |
5ul for 1st reaction + 5ul for re-trial reaction |
5ul + 5pmol/ul |
PCR product(over 500bp)
Purified / Unpurified |
Over 25ng/ul |
5ul for 1st reaction + 5ul for re-trial reaction |
5ul + 5pmol/ul |
PCR product(over 1000bp)
Purified / Unpurified |
30~60ng/ul |
5ul for 1st reaction + 5ul for re-trial reaction |
5ul + 5pmol/ul |
| BAC |
Over 500ng/ul |
5ul for 1st reaction + 5ul for re-trial reaction |
5ul + 10pmol/ul |
16S/18S/26S rRNA & ITS
region full Sequencing
(Agar Plate/Glycerol Stock) |
gDNA: 30-50ng |
Minimum volume of 50ul |
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| Difficult Sequencing |
100 ng/ul |
Minimum volume of 40ul |
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| Primer Walking |
8 µg
(for an insert size of up to 4kb) |
Minimum volume of 50ul |
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Note :
1. Primer Walking - Single Strand Sequencing: 1µg/1 kb insert. If insert size is longer than 4kb, clone is required in an agar stab culture status.
2. If you have a blood and tissue of insect/plant/animal or something, please contact us by email. info-sg@macrogen.com
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| a)
Sample for individual tube
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- General glycerol
stock/ Agar-stab/Agar-plate culture
at room temperature.
- 1.5mL Microcentrifuge Tube is recommended in a dried (lyophilized)
form or solution
..(Nuclease-free
TE or distilled water) at room
temperature..
- Free re-sequencing is included |
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b) 96 Well Plate : |
- 8 strip cap
is recommended in a dried (lyophilized)
form or solution (Nuclease-free
TE or distilled water)
.. at
room temperature.
- Re-sequencing is
additionally charged.
** In case of Multi primers for one plate **
Only two custom primers (or two universal primers) in tubes could be acceptable
for applying this Plate sequencing.
* Note:
- The well to well variation of the concentration of your samples is very important
to get
better results, please keep it as constant as possible.
- Please prepare samples to avoid
any well-to-well concentration
difference or size differencefor
quality results.
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| c)
Primer : |
- Primer : 5
pmol/ul in 20ul based on
5 samples
- Primer Tm is suitable for service : 55°C ~ 60°C
- Commonly used Universal primers are available for free of charge
(ex. M13F, T7, T3)
- Design and synthesize primers if you request. (US$0.25/base)
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It is possible to send either a stab or glycerol
culture with the selected E.coli clone,
the purified plasmid DNA or your unpurified
or purified PCR product.
Templates and primers must be provided in
DI water or 10mM Tris buffer, not in TE |
| d) To apply Plate sequencing : |
- 1 primer/ 1plate service : It is for covering one type of primer all through out the plate for clients who wish to run sequencing using
only primer through out the samples in the plate. If the client for example wish to run the sample plate with samples 1~96 using
primer 2, same price rate for 1Primer/1Plate service will be added.
- Multi primers/ 1plate service: This service is for clients who wish to run small numbers of different samples using different primers.
This service allows up to usage of 6 different primers under one plate service count covering 96 samples.
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