Order Guideline

 

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Order Guideline
You will find all involved information from placing an order through online to delivering samples to Macrogen.
If you have further questions, please do not hesitate to contact us at info-sg@macrogen.com
Log in
Access to “Make an order”
Complete Basic information
Complete Experiment Information by downloading Template File or by directly typing in direct online
Print out
Enclose it with samples
Ship out Samples

* Please do enclose printed order sheet together with your samples to avoid any delay of processing
   of your orders.
Template/Service Concentration Sample Volume Primer Volume
+ Concentration
Plasmid
(below 4kb )
100~150ng/ul 5ul for 1st reaction + 5ul for re-trial reaction 5ul + 5pmol/ul
Plasmid
(over 4kb)
200ng/ul 5ul for 1st reaction + 5ul for re-trial reaction 5ul + 5pmol/ul
PCR product(below 200bp)
Purified / Unpurified
10ng/ul 5ul for 1st reaction + 5ul for re-trial reaction 5ul + 5pmol/ul
PCR product(201bp~500bp)
Purified / Unpurified
20ng/ul 5ul for 1st reaction + 5ul for re-trial reaction 5ul + 5pmol/ul
PCR product(over 500bp)
Purified / Unpurified
Over 25ng/ul 5ul for 1st reaction + 5ul for re-trial reaction 5ul + 5pmol/ul
PCR product(over 1000bp)
Purified / Unpurified
30~60ng/ul 5ul for 1st reaction + 5ul for re-trial reaction 5ul + 5pmol/ul
BAC Over 500ng/ul 5ul for 1st reaction + 5ul for re-trial reaction 5ul + 10pmol/ul
16S/18S/26S rRNA & ITS
region full Sequencing
(Agar Plate/Glycerol Stock)
gDNA: 30-50ng Minimum volume of 50ul
Difficult Sequencing 100 ng/ul Minimum volume of 40ul
Primer Walking 8 µg
(for an insert size of up to 4kb)
Minimum volume of 50ul

Note :
1. Primer Walking - Single Strand Sequencing: 1µg/1 kb insert. If insert size is longer than 4kb, clone is required in an agar stab culture status.
2. If you have a blood and tissue of insect/plant/animal or something, please contact us by email. info-sg@macrogen.com


a) Sample for individual tube :
- General glycerol stock/ Agar-stab/Agar-plate culture at room temperature.
- 1.5mL Microcentrifuge Tube is recommended in a dried (lyophilized) form or solution
..(Nuclease-free TE or distilled water) at room temperature..
- Free re-sequencing is included
b) 96 Well Plate :
- 8 strip cap is recommended in a dried (lyophilized) form or solution (Nuclease-free TE or distilled water)
.. at room temperature.
- Re-sequencing is additionally charged.

** In case of Multi primers for one plate **
Only two custom primers (or two universal primers) in tubes could be acceptable for applying this Plate sequencing.

Note : For three or more primers, another paired primer plate with your sample plate should be provided. If not, your order will be regarded as Normal sequencing reactions.


c) Primer :
- Primer : 10 pmol/uL in 20µl based on 5 samples
- Primer Tm is suitable for service : 55°C ~ 60°C
- Commonly used Universal primers are available for free of charge (ex. M13F, T7, T3)
- Design and synthesize primers if you request. (US $0.60/base)
d) To apply Plate sequencing :
- 1 primer/1plate service :

- Multiple primers/ plate service :
  Up to 6 primers can be applied to this service (2~6 primers). Primers should be sent in separate EP tube
  in recommended concentrations and volume.

Note : Please note that if you send primers loaded in 96-well plate (mirror plate), then this will be regarded as “Standard sequencing service for single tube” instead of a plate.
- Shipping address :
Macrogen Inc.
# 1001, 254 Beotkkot-ro,
Geumcheon-gu, Seoul, 153-781 Rep. of KOREA

- Free shipping will be offered for samples of more than 20 reactions.

- Do not declare the value for custom over USD 10 for a convenience of custom clearance.

- Clearly state the postal code (153-781) on the airway bill for timely shipping.

- Please package your DNA samples securely and properly in order to avoid any potential damages in
   transit such as evaporation or contamination of samples during shipping.

- A printed copy of your order details must be enclosed in your sample package for our reference.

- Please enclose printed order sheet with samples to avoid any delay of processing of your orders.

- Dry Ice/ICEpack/ICE should not be put in samples which are dispatched to Macrogen Inc.


- Shipping address :
Macrogen Europe
Meibergdreef 31
1105 AZ Amsterdam Zuid-oost
Netherlands
- Please contact Macrogen Europe at europe@macrogen.com

- Please package your DNA samples securely and properly in order to avoid any potential damages in
  transit such as evaporation or contamination of samples during shipping.

- A printed copy of your order details must be enclosed in your sample package for our reference.
We have developed a new shipping protocol to make all users of Macrogen’s life easier!
This protocol will lead you through accomplishment of waybills and commercial invoices in few clicks!
We are hoping it will save some of your precious time!

Shall we begin the tutorial?




As your account carries all of your shipping information, sender information will be automatically submitted for you. If this address is not fitting for you, you will be allowed to save up to three different addresses. You could take usage of the saved addresses from the second time you take usage of the system.

When all three steps have been complete, simply call your local FedEx or DHL and request for pickup!


Not complicating at all, right?! Once you use it once, you will realize how easy shipping process has become now!

If you were ever worried about the safe arrival of your samples after dispatching, please be at eased now, as we will be keeping your samples monitored to promptly resolve any problem!

We promise you a continuous development to ease any of your difficulties.

Please continue to love Macrogen!
From your Loving International Sales Team.