| Sample Submission Guide |
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For more detailed explanations and recommendations
about the preparation and submission of samples,
please refer to the following link:
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 Next Generation Sequencing Sample Requirements
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DNA / RNA Quality
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a) DNA
All DNA sample(s) requirement...
-double-strand, non degraded, and containing
no particulate matter
-purified by column or gel purification protocols
-A260/280 ratio of 1.7 or above |
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b) RNA
All RNA sample(s) requirement...
-2100 Bioanalyzer RNA integrity Number (RIN) > 7
-28S rRNA : 18S rRNA = 2:1 ratio
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DNA / RNA / required Quantity
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a) 454 GS FLX (in EB Buffer)
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| HMW(>1.5kb) |
LMW(70~500bp) |
Mate pair (3~20Kb) |
| 0.5~1.0µg |
0.2µg |
5~30µg |
* For detailed sample requirements including Concentration, Purity, and Volume, refer to downloadable sample submission guideline.
In case of cDNA synthesis, tissue(>10g) or total RNA (>1µg) would be required. |
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| b) Illumina HiSeq2000/2500 & MiSeq (in EB buffer) |
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| Sample Type |
Total Quantity |
| gDNA (for rapid shotgun library) |
1.2µg (recommended) |
| gDNA (for Exome library) |
1.0~1.2µg (TruSeq / SureSelect kit) |
| 0.05µg (Nextra Rapid Exome Kit) |
| Total RNA (for cDNA / Strand-specific / small RNA library) |
>1.0µg (cDNA) / >4.0µg (Strand-specific) / 3.0µg (Small RNA) |
| mRNA (for cDNA / Strand-specific/small RNA library) |
>100ng (cDNA) / >100ng (Strand-specific) |
| small RNA |
entire fraction of small RNA purified from 1~10µg of total RNA |
* For detailed sample requirements including Concentration, Purity, and Volume, refer to downloadable sample submission guideline.
In case of ChIP Seq library, we use DNA 1000/7500 chip for normal PCR product, high sensitivity chip for very small amount of DNA fragment such as > 10ng of ChIPed DNA.
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Sample Submission Guide'
