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| Customized seq |
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For over a decade, Macrogen Inc. has been dedicating to provide
genomic solutions to researchers in all over the world with its advanced
DNA sequencing technology by using 23 ABI 3730XLs.
Thanks to over 10 years long know-how and large genomics
facility, we are able to provide our sequencing service for any other individuals
or institutes at the most competitive price. From small scale sequencing
reactions to large sequencing projects, we are ready to serve you with our
pleasure.
 Macrogen Customized Sequencing Brochure Download
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 Difficult sequencing
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" Sequencing of Difficult DNA templates" is
specified for templates which are not successful in normal sequencing
condition done by BigDye v.3.1, AB.
This service has different rate of price and
it is sequenced with more customized condition and
reagent. For more information,
please 
You may not get expected sequences depending
on sample characteristics.
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| 16s rRNA |
Starting from bacterial cell, we provide all inclusive
service performing DNA extraction, PCR,
sequencing
and assemble. 1,300bp or longer sequence is guaranteed for
domain bacteria.
For more information, please
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| 18S /ITS & 26S rRNA Full Sequencing |
It is getting more popular to define species by
using sequence information. This service is to find out rRNA sequence
by sequencing DNA corresponding to the rRNA.
In case of eukaryote, after rRNA full
sequencing, we can find out the similarity of customer's microorganism
with microogarnism in NCBI.
Starting from fungi cell, we provide all
inclusive service performing DNA extraction, PCR,
sequencing and assemble.
1. Analysis on 18S rRNA region sequences; length greater than 1,600 bp guaranteed.
2. Analysis on ITS region (18S prior to update); length greater than 500 bp guaranteed.
3. Analysis on rRNA gene(D1/D2/D3 region) length greater than 1,300 bp guaranteed.
For more information, please
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| Primer walking |
This service is to read 2~15kb of sequence with
PCR product or plasmid DNA that cannot be read
fully by single primer
extension sequencing. Once performing end sequencing with designated
primer by customer, we design internal primers with reference to end
sequence data.
Necessary walking continues with the same method
until the whole region is covered. About one
week is taken to read
1~1.5kb by primer walking.
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| Fragment Analysis |
Fragment Analysis encompasses
a wide variety of genotyping, DNA profiling, and mutation detection
techniques for medical, environmental, and agricultural research.
Macrogen Inc. provides the Fragment Analysis
based on our accumulated experience and know-how in genomics. In general,
it is used for amplified fragments' size check only. Since PCR
amplification
is not available through this service, fluorescent-labeled PCR products
should be
supplied as Microsatellite Analysis(VNTRs) samples.
For more information, please
Service Applications :
> Microsatellite Instability
> Amplified Fragment Length Polymorphism (AFLP)
Analysis
> Terminal Restriction Fragment Length Polymorphism
(T-RFLP) Analysis
> Relative Fluorescent Quantization - Loss of
Heterozygosity (LOH), Aneuploidy Assays,
and
Large Chromosomal Deletion Detection
> Sequence-related amplified polymorphism (SRAP)
provides a useful tool for estimation of
genetic diversity and phenetic relationships in natural
and domesticated populations.
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| Human ID |
Human ID Service is DNA analysis service which utilizes Multiplex-PCR Kit composed of STR(Short Tandem Rpeat) locus.
This service goes like below,
Human ID Service allows analysis
of optimal quality peaks using PCR products by configuring the proper concentration prior to processing under 3730XL.
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| Cloning
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Besides the main purpose of the cloning, we can
improve the sequencing results through this cloning service for contaminated
PCR products which usually show double peak in chromatogram.
This service goes like below,
- Step 1. Making plasmid by inserting a customer's
PCR products into vector (T-vector or Blunt-vector)
- Step 2. Inserting the plasmid into bacteria
cell for culture
- Step 3. Cell extraction for the plasmids
Please note that a customer shall inform us
that which type of DNA polymerase was used
in PCR
before sending samples because vector
for usage totally depends on the existence
of pfu-taq in DNA polymerase.
For more information, please |
| Human mtDNA Full Length Sequencing
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We provide direct sequencing of amplified 16,659 base pair human mitochondria DNA genes.
- Performing whole processes including PCR, sequencing, and assembling.
- Production of PCR products using 14 PCR primer sets, base sequence analysis using 39
sequencing primers, formation of full contig using each sequencing data.
- Time required : 14 days after Sample QC.
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| SNP |
SNP analysis service is highly flexible to any
specific project requirements.
SNPs (single nucleotide polymorphisms) occurs
every 100 to 300 bases along the human genome
and account for about 90% of all genetic variations. SNPs could predispose
people to disease
or influence their response to a drug.
We offer a complete package of SNP discovery
sequencing services by this step below,
PCR optimization => PCR amplification
=> Purification => Sequencing => SNP
discovery
We provide high-throughput SNP Analysis
by using ABI3730XL, automated DNA sequencer,
and the result will be available within
approximately two (2) weeks.
For more information, please
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| Library construction |
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