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What is Additional Order? |
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It’s a service which allows you to analyze DNA samples which have been sequenced previously with a new set of primer. For this additional order, the samples should be stored at Macrogen Inc. Please select the order type as “Additional Order” when you place the order online. Please note that it is a chargeable service as a new order not the re-sequencing order. |
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Library DNA QC Criteria |
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RNA QC Criteria |
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DNA QC Criteria |
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[Library QC method] Library Size Check |
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To verify the size of PCR enriched fragments, we check the template size distribution by running on a Agilent Technologies 2100 Bioanalyzer using a DNA 1000 chip.
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[DNA QC method] Assesing the condition of the DNA : Done by gel electrophoresis method. |
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Gel electrophoresis is a powerful means for revealing the condition (including the presence or absence) of DNA in a sample.
Impurities, such as detergents or proteins, can be revealed by smearing of DNA bands. RNA, which interferes with 260 nm readings, is often visible at the bottom of a gel.
A ladder or smear below a band of interest may indicate nicking or other damage to DNA. |
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[Analysis-Expression/miRNA] Why is there an overlapping gene? |
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Depending on chip platforms, multiple probes indicating a single gene can be implanted. The analysis is basically processed based on probe, but when analyzing gene, the overall signal is calculated by the average expression value of the probes. |
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[Analysis-TaqMan qPCR] Can you provide probe sequence information? |
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Due to the regulation of Applied Biosystems, TaqMan probe sequence information is not provided to the customers. |
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[Analysis-aCGH] Which Agilent CNV segmentation algorithm is used? |
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Agilent Genomic Workbench ADM-2 threshold 6 is used as a standard. |
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[Analysis-SNP] What does the basic service for SNP analysis includes? |
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'Basic service' for SNP analysis includes genotype data extraction, call rate, and standard QC. Other types of analysis, such as relatedness test by IBD score, stratification test by PCA, single based association, gene based association and linkage analysis, are considered as a 'customized analysis', which requires an additional cost. |
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[Analysis-SNP] What kind of files are required for designing a customized SNP chip? |
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If you provide us with any relevant information, such as rs# list, gene list, region list and sequence list, we will try to inform you about the possibility of chip customization using ADT (Assay Design Tool). |
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[Analysis-SNP] What are the procedures for designing VeraCode? |
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From 2014 onwards, VeraCode only manufacture 96,384 plex with 480 samples per order.
1. Select a plex to design (96 or 384 SNPs)
2. SNP Scoring for the candidate SNP list (selecting designable SNPs)
3. Confirm final OPA (Oligo Pool Assay)
4. Order the designed chip
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[Analysis-SNP] Once VeraCode chip designing is complete, how long would it takes to receive? |
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It takes approximately 7 weeks. Meanwhile, if you prepare the research team and samples, you will be able to manage the project more efficiently. |
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How do I use the extra sample or primer which was delivered along with the previous shipment? |
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In case of such primers which are not registered in previous orders but you sent us as extra ones for the purpose of further use, it will not appear on the list of stored primers since that information does not exist in our database but it is stored manually.
In such case, you may make an “Additional order” and register the primer as “enclosed” with a comment that the primer was already sent to Macrogen. Please do not forget to write the order number of the primer. You can also send us e-mail or LIMS message to ask for additional reactions. |
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How can I save the shipping fee if I need to send samples (or primers ) repeatedly for the held reactions? |
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Please send the held ones along with your next batch including 20 or more reactions.
Otherwise, your shipping is NOT free.
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I can't place an order by online due to computer system error. |
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To avoid any delay, please fill out the order sheet which can be down loaded from the below link and send the complete order sheet to us.
- Order sheet Download |
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Is there any restriction on reaction numbers per order? |
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If there are too many reactions in one order, you may get into trouble to check the result or download the data because of overload. Up to 384 reactions are recommended for one order. So please split your order into several orders in case of over 384 reactions. |
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Can I modify the sample name (or primer name) before sequencing? |
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Yes, you can modify the reactions information while the order status is shown as “Pending”. However, it is unable to modify any information when the order is confirmed. |
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Do I need to place an order separately depending on the sample condition? |
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Yes, each sample should be registered separately according to its sample type as long as the conditions of experiments between PCR product and plasmid are different, so please register samples separately if their types are different. |
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How do I pack the plates to avoid potential damages in transit? |
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Do I need to place an order before sending samples to Macrogen? |
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Yes, you should summit an order first before sending samples to us.
Otherwise, the experiment may NOT be performed.
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Free Shipping to Macrogen Inc. (Korea) or Macrogen Europe (The Netherlands) |
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Macrogen Inc.(Korea) pay shipping fee for more than 20 reactions's order.
Our account number of Fedex(preferred), DHL or TNT should clearly be written and it should be checked as reciever's payment on the airway bill. We are not responsible for paying wrongly charged invoice.
It is room temperature packing only and should be less than 1kg. (Not in a big box.) If you want to pack your samples with dry ice/ice pack, shipping fee is not covered by Macrogen Inc.(Korea).
**Free shipping for European express sequencing service is also applied, so please contact Macrogen Europe at europe@macrogen.com. |
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Glycerol Concentration |
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I need to know how should I send my cells for sequencing. What percentage of Glycerol should I use in the samples.
=> 1:1 mix up with 30% glycerol stock to make 15% of final glycerol concentration would be fine. |
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Do you synthesize a primer? |
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Yes. We do synthesize a primer.
Macrogen offers the oligonucleotide DNA synthesis service applying the cutting-edge technology and the state-of-the-art facility.
We also provide free delivery for your oligonucleotide DNA through express courier service if the order is over 300 mers.
Please click the link below for more information about the oligonucleotide DNA synthesis service.
http://dna.macrogen.com/eng/support/news/news_view.jsp?board_number=5981
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Will you keep my samples? |
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We can keep leftover of DNA and primers for one month ~ three months upon receipt. If you want to sequence among them, please place an order from "Additional Order" menu. Or, you may just give us a comment when and from whom samples were delivered here with sequencing information. |
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May I place an order in Maryland Branch? |
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You may also use our Maryland branch's facility. It has the same quality of sequencing skills. But it is independent from Seoul, Korea office and the price is little bit different from Korea headquarter's.
You may contact US branch and get more information.
9700 Great Seneca Hwy
Rockville MD
20850 USA
Tel : 301-251-1007
Fax : 301-251-4006
customer@macrogenusa.com |
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Is there minimum number of samples in sequencing order? |
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No, there is no such minimum number or maximum number of sequencing reactions. Just we recommend you send DNAs for at least 20 sequencing reactions to save your shipping fee. |
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Do I need to use on-line system all the time? |
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On-line system is made for the ease and accuracy of order, so we recommend you to use the system. It is much more convenient because you will be able to order online and retrieve the result online as well as leave a message in no time. |
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What is the average read length? |
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We read approximately ~850bp per reaction, and 650bp of them has Phred 20 or higher quality. |
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What kind of machines you use? |
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We use ABI3730XL |
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What kind of computer system is required for on-line system? |
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The system is designed to be compatible with all of the systems including PC, Mac, and many other conventional operating system. |
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Universal primers available in Macrogen |
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| Primer Name | Sequence | Base | | Bluescript SK | CGCTCTAGAACTAGTGGATC | 20 | | EBV-RP | GTGGTTTGTCCAAACTCATC | 20 | | KAN2-FP | ACCTACAACAAAGCTCTCATCAACC | 25 | | KAN2-RP | GCAATGTAACATCAGAGATTTTGAG | 25 | | M13-FP | TGTAAAACGACGGCCAGT | 18 | | pBacPAC-RP | GTCTGTAAATCAACAACGC | 19 | | pBAD-FP | ATGCCATAGCATTTTTATCC | 20 | | pDONOR-FP | TAACGCTAGCATGGATCTC | 19 | | pEGFP_N | CCGTCCAGCTCGACCAG | 17 | | pEGFP-FP | TTTAGTGAACCGTCAGATC | 19 | | pEGFP-RP | AACAGCTCCTCGCCCTTG | 18 | | pESP-RP | TCCAAAAGAAGTCGAGTGG | 19 | | pET-24a | GGGTTATGCTAGTTATTGCTCAG | 23 | | pET-RP | CTAGTTATTGCTCAGCGG | 18 | | pMalE | TCAGACTGTCGATGAAGC | 18 | | pREP-fwd | GCTCGATACAATAAACGCC | 19 | | 35S-A | AAGGGTCTTGCGAAGGATAG | 20 | | 35S-B | AGTGGAAAAGGAAGGTGGCT | 20 | | AD Reverse | AGATGGTGCACGATGCACAG | 20 | | CYC1 Reverse | GCGTGAATGTAAGCGTGAC | 19 | | DsRed1-C | AGCTGGACATCACCTCCCACAACG | 24 | | DsRed1-N | GTACTGGAACTGGGGGGACAG | 21 | | EGFP-C | CATGGTCCTGCTGGAGTTCGTG | 22 | | EGFP-N | CGTCGCCGTCCAGCTCGACCAG | 22 | | GAL1 Forward | AATATACCTCTATACTTTAACGTC | 24 | | U-19mer Primer | GTTTTCCCAGTCACGACGT | 19 | | T7 EEV | ATGTCGTAATAACCCCGCCCCG | 22 | | Bluescript KS | TCGAGGTCGACGGTATC | 17 | | pFastBac Forward | GGATTATTCATACCGTCCCA | 20 | | pFastBac Reverse | CAAATGTGGTATGGCTGATT | 20 | | AOX1 Forward | GACTGGTTCCAATTGACAAGC | 21 | | AOX1 Reverse | GCAAATGGCATTCTGACATCC | 21 | | a-Factor | TACTATTGCCAGCATTGCTGC | 21 | | STag 18mer Primer | GAACGCCAGCACATGGAC | 18 | | MT Forward | CATCTCAGTGCAACTAAA | 18 | | QE Promoter | CCGAAAAGTGCCACCTG | 17 | | pRH Forward | CTGTCTCTATACTCCCCTATAG | 22 | | pRH Reverse | CAAAATTCAATAGTTACTATCGC | 23 | | SV40-pArev | CCTCTACAAATGTGGTATGG | 20 | | SV40-Promoter | GCCCCTAACTCCGCCCATCC | 20 | | pTrcHis Forward | GAGGTATATATTAATGTATCG | 21 | | ITS1 | TCCGTAGGTGAACCTGCGG | 19 | | ITS2 | GCTGCGTTCTTCATCGATGC | 20 | | ITS3 | GCATCGATGAAGAACGCAGC | 20 | | ITS4 | TCCTCCGCTTATTGATATGC | 20 | | pJET1.2F | CGACTCACTATAGGGAGAGCGGC | 23 | | pJET1.2R | AAGAACATCGATTTTCCATGGCAG | 24 | | T7 | AATACGACTCACTATAG | 17 | | T7terminator | GCTAGTTATTGCTCAGCGG | 19 | | T7promoter | TAATACGACTCACTATAGGG | 20 | | T3 | ATTAACCCTCACTAAAG | 17 | | SP6 | ATTTAGGTGACACTATAG | 18 | | M13F-pUC(-40) | GTTTTCCCAGTCACGAC | 17 | | M13R-pUC(-40) | CAGGAAACAGCTATGAC | 17 | | M13F | GTAAAACGACGGCCAGT | 17 | | M13R | GCGGATAACAATTTCACACAGG | 22 | | pGEX5 | GGCAAGCCACGTTTGGTG | 18 | | pGEX3 | GGAGCTGCATGTGTCAGAGG | 20 | | 27F | AGAGTTTGATCMTGGCTCAG | 20 | | 1492R | TACGGYTACCTTGTTACGACTT | 22 | | 518F | CCAGCAGCCGCGGTAATACG | 20 | | 800R | TACCAGGGTATCTAATCC | 18 | | BGH-R | TAGAAGGCACAGTCGAGG | 18 | | CMV-F | CGCAAATGGGCGGTAGGCGTG | 21 | | RVprimer3 | CTAGCAAAATAGGCTGTCCC | 20 | | RVprimer4 | GACGATAGTCATGCCCCGCG | 20 | | GLprimer1 | TGTATCTTATGGTACTGTAACTG | 23 | | GLprimer2 | CTTTATGTTTTTGGCGTCTTCCA | 23 | | pQE-F | CCCGAAAAGTGCCACCTG | 18 | | pQE-R | GTTCTGAGGTCATTACTGG | 19 | | Gal4AD | TACCACTACAATGGATG | 17 | | pBAD-F | ATGCCATAGCATTTTTATCCA | 21 | | pBAD-R | GATTTAATCTGTATCAGG | 18 | | EGFP-CF | AGCACCCAGTCCGCCCTGAGC | 21 | | EGFP-CR | CGTCCATGCCGAGAGTG | 17 | | EGFP-NR | CGTCGCCGTCCAGCTC | 16 |
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What are your preferred methods of purification? |
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We do not have any special preference for purification kit. Conventional column-type purification is good as well as the Exo-SAP purification. |
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How much is it to run the samples with unsatisfactory results again? |
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Retrial for failed ones are provided for free for once.
It is free only when samples and primers left sufficiently and it should be the same DNA and primer match from the first trial.
Note : Free retrial is Not available for Plate sequencing service and 1primer/1plate service. |
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Only a partial number of samples were processed. |
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We assign all the samples in 384-well plates and proceed with our machines, and in some cases, your samples can be divided in two different plates. If this happens, you will be receiving the results in two different batches. Also, if you've requested samples for PCR prufication along with samples for ready-to-sequence, they can be divided and sent separetely. We will be giving all the results in the end, and if you don't receive the other part of the results within one week, please contact us immedaitely. |
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How long does it take to get my results back? |
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Within 48 hours from the date of sample arrival, the results shall be sent to you.
It may take 3~4 more days in case of primer synthesis or purification. |
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I cannot unzip the result file. |
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If you are using MAC, not PC, we can provide *.sit compressed file as well. Please request us for this. |
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I haven't received my results yet. |
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We generally send the results with 48 hours from the date of samples reception, and if you haven't received the results by then, please contact us immediately. |
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I cannot open AB1 files. |
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Our AB1 files can be opened by conventional softwares such as BioEdit, Chromas, Finch TV, Vector NTI, and so many applications. For those customers without these softwares, we provide Acrobat PDF files as well. |
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How long the results are stored in your web page? |
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The results are stored for three months, after then results will be discarded. We recommend you to download the results in your secure location as soon as they become available in the web. |
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How can I order online? |
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I haven't received the arrival notice yet. What happened? |
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Arrival notice is usually sent a little bit later when all the samples are properly arranged. But if you haven't received our arrival notice for one week, please contact us immediately with the Fedex tracking number. Probably there would be a problem in unidentified sender and orderer. |
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How long are my samples kept in freezer of Macrogen? |
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Basically we keep them for one month, and on special request, we extend this to be three months. |
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Should I send the primers along with the template DNA? |
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We recommend the primers to be sent in separate tubes with template DNA so that we can adjust the amount of primer. |
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Could you please advise when samples will be picked up? |
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We currently provide sample-pickup service in a couple of region in the world, and please contact us for the pickup in your location. Usually, our representative visit your lab on designated day to pick up your samples.
Currently Available Institution :
*Melbourne University(Australia): Friday
*Kasetsart University/Chulalongkorn University/Mahidol University(Thailiand): Friday
*Buenos Aires(Argentina): Friday
- Please simply phone to +54 11 4331 4400 (Eda Lee)
- Send an e-mail: lee@ubiqgroup.com, gio@ubiqgroup.com,
eda@lkglobal.com.ar (Contact in Argentina)
*Bogota (Colombia): Friday
- Please simply phone to +57 310 817 1550 (Fernando Lee)
- Send an e-mail: lee@ubiqgroup.com, gio@ubiqgroup.com,
eda@lkglobal.com.ar (Contact in Argentina)
*University of Copenhagen(Denmark): Tuesday, Thursday
*Hvidovre Hospital/Statens Serum Institute(Denmark): Thursday
We are going to expand this pickup service in near future. |
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Concentration of plasmid / PCR product |
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Minimum 20ul of 100ng/ul(plasmids, unpurified PCR products) or 50ng/ul(purified PCR products) are required for a couple of reactions. |
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Do I need to put dry ice or any chilling material in the parcel? |
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Dry Ice is NOT required. It is dangerous goods in customs clearance and it will take longer time to deliver. And we will not pay for shipping for package with dry ice. |
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Fedex is not available in my location. How can I send the samples? |
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We recommend Fedex most. But in some places, it may not be an easy choice.
You can use TNT or DHL. Please contact us(info@macrogen.com or europe@macrogen.com) for trying other courier service. |
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How many samples should I send to be eligible for the free shipping by Fedex? |
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More than 20 reactions(or equivalent number of samples) shall be eligible for free shipping to Macrogen Inc.(Korea) or Macrogen Europe (The Netherlands). You may refer our account number and check the bill as recipient's payment.
You may collect your colleagues' samples to make up 20 or more reactions and save your shipping fee.
We never reimburse the shipping fee but you should check the bill as recipient's payment. |
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Shipment Address |
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Shipping address for Value sequencing service is :
Macrogen Inc.
#1001, 254 Beotkkot-ro,
Geumcheon-gu, Seoul, 08511
Rep. of KOREA
Shipping address for European express sequencing service is :
Macrogen Europe
Meibergdreef 31
1105 AZ Amsterdam Zuid-oost
Netherlands |
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